Guigen Ma,Fang Yan,Bin Ren,Zhenwan Lu,Hao Xu,Fangxi Wu, Shaofang Li, Daowen Wang, Xueping Zhou, Huanbin Zhou.LbCas12a nuclease-mediated tiling deletion for large-scale targeted editing of non-coding regions in rice.
Summary
NTD method is the application of LbCas12a in the engineering of rice endogenous gene. Guided by libraries of crRNAs tiling on NCRs of SD1 , 6 mutants with quantitative variations of rice height were generated and analyzed for the association between genotype and phenotype. Totally, NTD is a promising tool for molecular rice breeding.
Plant Communications，IF=10.8
https://doi.org/10.1016/j.xplc.2024.100815