Xiao Xu, Xuetao Shi, Xiaoman You, Zeyun Hao, Ruyi Wang , Min Wang, Feng He, Shasha Peng, Hui Tao, Zheng Liu, Jisong Wang, Chongyang Zhang, Qin Feng, Weixun Wu, Guo-Liang Wang, Yuese Ning. A pair of E3 ubiquitin ligases control immunity and flowering by targeting different ELF3 proteins in rice.
Developmental Cell ，2024，https://doi.org/10.1016/j.devcel.2024.06.013
Summary
The ubiquitin-proteasome system (UPS) plays crucial roles in cellular processes including plant growth, development, and stress responses. In this study, we report that a pair of E3 ubiquitin ligases, AvrPiz-t-interaction protein 6 (APIP6) and IPA1-interaction protein 1 (IPI1), intricately target early flowering3 (ELF3) paralogous proteins to control rice immunity and flowering. APIP6 forms homo-oligomers or hetero-oligomers with IPI1. Both proteins interact with OsELF3-2, promoting its degradation to positively control resistance against the rice blast fungus (Magnaporthe oryzae). Intriguingly, overexpression of IPI1 in Nipponbare caused significantly late-flowering phenotypes similar to the oself3-1 mutant. Except for late flowering, oself3-1 enhances resistance against M. oryzae. IPI1 also interacts with and promotes the degradation of OsELF3-1, a paralog of OsELF3-2. Notably, IPI1 and APIP6 synergistically modulate OsELF3s degradation, finely tuning blast disease resistance by targeting OsELF3-2, while IPI1 controls both disease resistance and flowering by targeting OsELF3-1. This study unravels multiple functions for a pair of E3 ligases in rice.
Developmental Cell，IF=10.7
https://www.sciencedirect.com/science/article/pii/S1534580724003915?via%3Dihub