中文名：李世访EDUCATION:
Ph.D. 1993, Agriculture Biology, Hokkaido University, Japan.
M.S. 1990, Agriculture Biology, Hokkaido University, Japan.
B.S. 1986, Plant Protection, Jilin Agricultural University, China.
WORKING EXPERIENCE:
Professor, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, China, 2007-present.
Associate Professor, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, China, 2000-2005.
Postdoctor, The University of Tokyo, Hirosaki University, Japan, 1994-2000.
Research Area：
Fruit trees viral diseases were less studied compared with viral diseases infecting main crops in China. The research activity of Viral Diseases of Cash Crops Group (VDCCG) focuses on viruses, and viroids the smallest plant viral pathogen infecting fruit trees and other perennial plants. The overall objective is to develop new approaches for the discovery, characterization, detection of these agents, and for the analysis of their diversity. High throughput sequencing technologies combined with other traditional detection methods developed for diagnostic purposes, epidemiological analysis and also for the interaction between plant and viral agents.
Publications：
[1] Liang Pengbo, Beatriz Navarro, Zhang Zhixiang, Wang Hongqing, Lu Meiguang, Xiao Hong, Wu Qingfa, Zhou Xueping, Di Serio Francesco, Li Shifang. Identification and characterization of a novel geminivirus with monopartite genome infecting apple trees. Journal of General Virology. doi: 10.1099/vir.0.000173.(2015)
[2] Ma Yuxin, Navarro Beatriz, Zhang Zhixiang, Lu Meiguang, Zhou Xueping, Chi Shengqi, Di Serio Francesco, Li Shifang. Identification and molecular characterization of a novel monopartite geminivirus associated with mulberry mosaic dwarf disease. Journal of General Virology. doi: 10.1099/vir.0.000175.(2015)
[3] Mijit Mahmut, Li Shifang, Zhang Sheng, Zhang Zhixiang. Frist report of Fig masaic virusinfecting common Fig(Ficus carica)in China. Plant Disease. 99(3):422.3-422.3.(2015)
[4] Zhang Zhixiang, Qi Shuishui, Tang Nan, Zhang Xinxin, Chen Shanshan, Zhu Pengfei, Ma Lin, Cheng Jinping, Xu Yun, Lu Meiguang, Wang Hongqing, Ding Shouwei, Li Shifang, Wu Qingfa. Discovery of replicating circular RNAs by RNA-Seq and computational algorithms.PLoS Pathogens. 10(12): e1004553. doi:10.1371/journal.ppat.1004553.(2014)
[5] Jiang Dongmei, Gao Rui, Qin Lv, Wu ZuJian, Xie LianHui, Hou Wanying, Li Shifang.Infectious cDNA clones of four viroids in Coleus blumei and molecular characterization of their progeny. Virus research. 108:97-101.(2014)
[6] Qin Lü, Ma Yuxin, Liang Pengbo, Tan Zhongyang, Li Shifang. Differential distributions of mononucleotide repeat sequences in 256 viral genomes and its potential implications. Gene. 544, 159-164.(2014)
[7] Qin Lü , Zhang Zhixiang, Zhao Xiangyan, Wu Xiaolong, Chen Yubao, Tan Zhongyang, Li Shifang. Survey and analysis of simple sequence repeats (SSRs) present in the genomes of plant viroids. FEBS Open Biology. 4, 185-189.(2014)
[8] He Zhen, Li Wenfeng, Yasaka Ryosuke, Huang Yingkun, Zhang Zhixiang, Ohshima Kazusato, Li Shifang Molecular variability of sugarcane streak mosaic virus in China based on an analysis of the P1 and CP protein coding regions. Archives of Virology. 159:1149-1154. (2014)
[9] Ge Beibei, He Zhen, Zhang Zhixiang, Wang Hongqing, Li Shifang. Genetic variation in potato virus M isolates infecting pepino (Solanum muricatum) in China. Archives of Virology. 159, 3197-3210.(2014)
[10] Di Serio Francesco, Flores Ricardo, Verhoeven Jacobus Th. J, Li Shifang, Pallas Vicente, Randles John W, Sano Teruo, Vidalakis G, Owens Robert. Current status of viroid taxonomy.Archives of Virology. 159, 3467-3478.(2014)
[11] Chen Shanyi, Zhou Ying, Ye Ting, Hao Lu, Guo Liyun, Fan Zaifeng, Li Shifang, Zhou Tao. Genetic variation analysis of apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China. Archives of Virology. 159, 1431-1438.(2014)
[12] Ge Beibei, Li Qian, Liu Guojie, Lu Meiguang, Li Shifang, Wang Hongqing. Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR. Archives of Virology. 158:1181-1187.(2013)
[13] Wang Lihui, Jiang Dongmei, Niu Feiqing, Lu Meiguang, Wang Hongqing, Li Shifang. Complete nucleotide sequences of two isolates of cherry green ring mottle virus from peach (Prunus persica) in China. Archives of Virology. 158:707-710.(2013)
[14] Yu Yun, Zhao Zhe, Jiang Dongmei, Wu Zijian, Li Shifang. A one-step multiplex RT-PCR assay for simultaneous detection of four viruses that infect peach. Letters in Applied Microbiology. 57:350-355.(2013)
[15] Jiang Dongmei, Li Shifang, Fu Fanghong, Wu Zujian and Xie Lianhui. First report of Coleus blumei viroid 5 from Coleus blumei in India and Indonesia. Plant Disease. 97: 561.(2013)
[16] Jiang Dongmei, Hou Wanying, Sano Teruo, Kang Ni, Qin Lü, Wu Zujian, Xie Lianhui and Li Shifang. Rapid detection and identification of viroids in the genus Coleviroid using a universal probe. Journal of Virological Methods. 187: 321-326.(2013)
[17] Yu Yun, Zhao Zhe, Qin Lv, Zhou Ying, Fan Hongyan, Zhang Zhixiang, Wu Zujian and Li Shifang. Incidence of major peach viruses and viroids in China. Journal of Plant Pathology. 95(3): 603-607.(2013)
[18] Ye Ting, Chen Shanyi, Wang Rong, Hao Lu, Chen Hui, Wang Nian, Guo Liyun, Fan Zaifeng,Li Shifang, Zhou Tao. Identification and molecular characterization of Apple dimple fruit viroid in China. Journal of Plant Pathology. 95(3): 637-641.(2013)
[19] Zhao Zhe, Yu Yun, Zhang Zhixiang, Liang Pengbo, Ma Yuxin, Li Shifang, Hongqing Wang. A duplex, SYBR Green I-based RT-qPCR assay for the simultaneous detection of Apple chlorotic leaf spot virus and Cherry green ring mottle virus in peach. Virology Journal. 10: 255.(2013)
[20] Zhang Yongjiang, Yin Jun, Jiang Dongmei, Xin Yanyan, Ding Fang, Deng Ziniu, Wang Guoping, Ma Xianfeng, Li Fang, Li Guifen, Li Mingfu, Li Shifang and Zhu Shuifang. A universal oligonucleotide microarray with a minimal number of probes for the detection and identification of viroids at the genus level. PLoS ONE. 8(5): e64474.(2013)
[21] Jiang Dongmei, Sano Teruo, Tsuji Masaharu, Araki Hiroyuki, Kyota Sagawa, Purushothama Charith Raj Adkar, Zhang Zhixiang, Guo Rui, Xie Lianhui, Wu Zujian, Wang Hongqing andLi Shifang. Comprehensive diversity analysis of viroids infecting grapevine in China and Japan. Virus Research. 169: 237-245.(2012)
[22] Niu Feiqing, Pan Song, Wu Zujian, Jiang Dongmei and Li Shifang. Complete nucleotide sequences of the genomes of two isolates of Apple chlorotic leaf spot virus from peach (Prunus persica) in China. Archives of Virology. 157(4): 783-786.(2012)
[23] Zhang Zhixiang, Zhou Ying, Guo Rui, Mu Lingxiao, Yang Yuanai, Li Shifang and Wang Hongqing. Molecular characterization of Chinese Hop stunt viroid isolates reveals a new phylogenetic group and possible cross transmission between grapevine and stone fruits.European Journal of Plant Pathology. 134: 217-225.(2012)
[24] Zhang Zhixiang, Peng Shan, Jiang Dongmei, Pan Song, Wang Hongqing and Li Shifang. Development of a polyprobe for the simultaneous detection of four grapevine viroids in grapevine plants. European Journal of Plant Pathology. 132: 9-16.(2012)
[25] Niu Feiqing, Pan Song, Wu Zujian, Jiang Dongmei and and Li Shifang. First report of Apricot pseudo-chrolotic leaf spot virus infection of peach in China. Journal of Plant Pathology. 94: S4.102(2012)
[26] Lu Wenxia, Zhang Zhixiang, Xu Pinsan, Liu Shengxue, Wang Hongqing, Jiang Dongmei andLi Shifang. Simultaneous detection of three viroid species infecting hops in China by multiplex RT-PCR. Journal of Phytopathology. 160(6): 308-310.(2012)
[27] Fu Fanghong, Li Shifang, Jiang Dongmei, Wang Hongqing, Liu Aiqin and Sang Liwei. First report of Coleus blumei viroid 2 from commercial coleus in China. Plant Disease. 95(4): 494.(2011)
[28] Zhang Zhixiang, Pan Song and Li Shifang. First report of Chrysanthemum chlorotic mottle viroid in chrysanthemum in China. Plant Disease. (95): 1320.(2011)
[29] Li Wenfeng, He Zhen, Li Shifang, Huang Yingkun, Zhang Zhixiang, Jiang Dongmei, Wang Xiaoyan and Luo Zhiming. Molecular Characterization of a new tentative strain of Sugarcane streak mosaic virus (SCSMV). Archives of Virology. 156: 2101-2104.(2011)
[30] Jiang Dongmei, Wu Zujian, Xie Lianhui, Sano Teruo and Li Shifang. Sap-direct RT-PCR for the rapid detection of coleus blumei viroids of the genus Coleviroid from natural host plants.Journal of Virological Methods. 174: 123-127.(2011)
[31] He Zhen, Jiang Dongmei, Liu Aiqin, Sang Liwei, Li Wenfeng and Li Shifang. The complete sequence of Cymbidium mosaic virus from Vanilla fragrans in Hainan, China. Virus Genes. 42: 440-443.(2011)
[32] Jiang Dongmei, Li Shifang, Fu Fanghong, Wu Zujian and Xie Lianhui. First report occurrence of coleus blumei viroid 3 from coleus blumei China. Journal of Plant Pathology. 93(4): S4.82.(2011)
[33] Kawaguchi-Ito Yoko, Li Shifang, Tagawa Masaya, Araki Hiroyuki, Goshono Masafumi, Yamamoto Shingen, Tanaka Mayumi, Narita Masako, Tanaka Kazuaki, Liu Shengxue, Shikata Eishiro and Sano Teruo. Cultivated grapevines represent a symptomless reservoir for the transmission of Hop stunt viroid to hop crops: 15 years of evolutionary analysis. PLoS ONE. 4(12): e8386.(2009)
[34] Hou Wanying, Sano Teruo, Li Feng, Wu Zujian, Li Li and Li Shifang. Identification and characterization of a new Coleviroid (CbVd-5). Archives of Virology. 154: 315-320.(2009)
[35] Hou Wanying, Li Shifang, Wu Zujian, Jiang Dongmei, Sano Teruo. Coleus blumei viroid 6: a new tentative member of the genus Coleviroid derived from natural genome shuffling.Archives of Virology. 154: 993-997.(2009)
[36] Zhang Wenwei, Cheng Zhuomin, Xu Lei, Wu Maosen, Waterhouse Peter, Zhou Guanghe andLi Shifang. The complete nucleotide sequence of the barley yellow dwarf GPV isolate from China shows that it is a new member of the genus Polerovirus. Archives of Virology. 154: 1125-1128.(2009)
[37] Jiang Dongmei, Guo Rui, Wu Zujian, Wang Hongqing and Li Shifang. Molecular characterization of a member of a new species of grapevine viroid. Archives of Virology. 154: 1563-1566.(2009)
[38] Zhang Benli, Liu GuangYao, Liu Chunqin, Wu Zujian, Jiang Dongmei, Li Shifang. Characterisation of Hop stunt viroid (HSVd) isolates from jujube trees (Ziziphus jujuba). European Journal of Plant Pathology. 125: 665-669.(2009)
[39] Mu Lingxiao, Li Shifang, Wu Yuanhua and Dong Yafeng. First report of Hop stunt viroid isolates from almond tree in China. Journal of Plant Pathology. 91: S4.102(2009)
[40] Jiang Dongmei, Peng Shan, Wu Zujian, Cheng Zhuomin and Li Shifang. Genetic diversity and phylogenetic analysis of Australian grapevine viroid (AGVd) isolated from different grapevines in China. Virus Genes. 38: 178-183.(2009)
[41] Jiang Dongmei, Zhang Zhixiang, Wu Zujian, Guo Rui, Wang Hongqing, Fan Peige and Li Shifang. Molecular characterization of Grapevine yellow speckle viroid-2 (GYSVd-2). Virus Genes. 38: 515-520.(2009)
[42] Liu Shengxue, Li Shifang, Zhu Jianbo, Xiang Benchun and Cao Lianpu. First report of hop latent viroid (HLVd) in China. Plant Pathology. 57: 400.(2008)
[43] Guo Lihua, Liu Shengxue, Wu Zujian, Mu Lingxiao, Xiang Benchun and Li Shifang. Hop stunt viroid (HSVd) newly reported from hop in Xinjiang, China. Plant Pathology. 57: 764.(2008)
[44] Yang Yuanai, Wang Hongqing Cheng Zhuomin and Li Shifang Molecular variability of hop stunt viroid: Identification of a unique 312 nucleotides variant with a tandem 15 nucleotides repeat from naturally infected plum tree. Biochemical Genetics. 46: 113-123.(2008)
[45] Li Shifang, Guo Rui, Peng shan and Sano Teruo. Grapevine yellow speckle viroid 1 and grapevine yellow speckle viroid-2 isolates from China. Journal of Plant Pathology. 89: S572.(2007)
[46] Guo Rui, Sano Teruo, Cheng Zhuomin and Li Shifang. Detection of Australian grapevine viroid in a grapevine more than 100 years old in Xinjiang, China. Plant Pathology. 56: 339.(2007)
[47] Yang Yuanai, Wang Hongqing, Cheng Zhuomin and Li Shifang. First report of Hop stunt viroid from plum in China. Plant Pathology. 56: 339.(2007)
[48] Li Shifang, Guo Rui, Tsuji Masaharu, Sano Teruo. Two grapevine viroids in China and the possible detection of a third. Plant Pathology, 55(4), 564-564.(2006)
[49] Li Shifang, Su Qianfu, Guo Rui, Tsuji Masaharu, Sano Teruo. First report of Coleus blumei viroid from coleus in China. Plant Pathology, 55(4), 565-565.(2006)
[50] Zhou Ying, Guo Rui, Cheng Zhuomin, Sano Teruo, Li Shifang. First report of Hop stunt viroid from Prunus persica with dapple fruit symptoms in China. Plant Pathology, 55(4), 564-564.(2006).
[51] Yan Fei, Zheng Yinying, Zhang Wenwei, Xiao Hong, Li Shifang, Cheng Zhuomin. Obtained transgenic wheat expressing pac1 mediated by Agrobacterium is resistant against Barley yellow dwarf virus-GPV. Chinese Science Bulletin, 51(19), 2362-2368.(2006)
[52] Yang Yuanai, Wang Hongqing, Guo Rui, Cheng Zhuomin, Li Shifang, Sano Teruo. First Report of Hop stunt viroid in Apricot in China. Plant Disease, 90(6), 828-828.(2006)
