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The base for biological control of locusts with Metarhizium

 

——National Demonstration Base for Introducing Foreign Intelligence

 

  The base for biological control of locusts with Metarhizium was established in 2003. This base focused on the study: develop the reasonable synergist of Metarhizium, improve the technology of biological anti-locust by Metarhizium, and increase the efficiency of biological control of locusts with Metarhizium. These studies were supported by the “The Study in the Development and Application of Synergist Type of Metarhizium anisopliae and Sublethal Concentration of the Chemical Compound, Y20140326002” in 2014. We demonstrated 100,000 Mu of biological control of locusts with Metarhizium in Inner Mongolia Autonomous Region of China; trained six graduate students, two PhD and two postdoctors; published 8 research papers 8 (SCI: 1); and held 2 training meetings in base in 2014.
  1.The co-toxicity between chemical pesticide and Metarhizium
  In the laboratory, we found that 0.5 %-1.5 % (w/v) cypermethrin could increase the toxicity of Metarhizium to Locusta migratoria manilensis, and the co-toxicity coefficient between chlorantraniliprole and Metarhizium was higher than 120 when chlorantraniliprole was mixed with Metarhizium according to the proportion 2:8 and 5:5.
  2. The biochemical mechanism in the co-toxicity between chlorantraniliprole and Metarhizium
  The results showed that M. anisopliae increased the sensitivity of multi-function oxidase and inhibited the detoxification of glutathione-S-transferase of L. migretoria to chlorantraniliprole and chlorantraniliprole inhibited the activity of esterases and phenol oxidase (PO) of L. migretoria to M. anisopliae during the initial period of M. anisopliae infection, and enhanced the activity of acetylcholinesterase and inhibited the activities of aryl acylamidase, chitinase, catalase, peroxidase and superoxide dismutase (SOD) of L.migretoria by combined action of M. anisopliae and chlorantraniliprole at late period of M. anisopliae infection.
  3. The co-toxicity to grasshopper of chlorantraniliprole and Metarhizium in grassland
  In the grassland, the control of the combination both M. anisopliae and chlorantraniliprole to grasshopper was better than M. anisopliae or chlorantraniliprole. The density of insect was decreased 70%, 72% and 85% by M. anisopliae, chlorantraniliprole and the combination both M. anisopliae and chlorantraniliprole respectively when the density of insect increased 20% in control. And, the combination both M. anisopliae and chlorantraniliprole overcome the shortcoming of M. anisopliae in controlling grasshopper that was slow in insecticidal, low in control effect and difficult for high-density locust prevention.
  4. Demonstration and promotion the control of the combination both M. anisopliae and chlorantraniliprole to grasshopper.
  In order to promote the control of the combination both M. anisopliae and chlorantraniliprole to grasshopper, we hold the training courses and the scene in Taipusiqi, Xilin Gol League, Inner Mongolia in 9-11 July 2014 that was co-organized by Xilin Gol Grassland workstation of Inner Mongolia. This meeting archived good social benefit by training more than 100 farmers and herdsmen, and the demonstration of 100,000 Mu archived good economic benefit.

 


The base of biological control of locusts with Metarhizium

 


The grasshopper infected by Metarhizium anisopliae

 


The aircraft sowing Metarhizium anisopliae

 


Products of Metarhizium anisopliae

 


Granules of Metarhizium anisopliae

 




Copyright:Institute of Plant Protection,Chinese Academy of Agricultural Sciences 

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