http://www.sklbpi.cn/jinqifabiaolunwen/750.html
Abstract
MicroRNAs
(miRNAs) are short single-stranded non-coding RNAs that regulate gene
expression, particularly during development. In this study, 345 miRNAs were
identified from the English green aphid, Sitobion avenae (F.), of which 168 were
conserved and 177 were S. avenae-specific. Quantitative comparison of miRNA
expression levels indicated that 16 and 12 miRNAs were significantly upregulated
in winged and wingless S. avenae small RNA libraries, respectively. Differential
expression of these miRNAs was confirmed by real-time quantitative RT-PCR
validation. The putative transcript targets for these candidate miRNAs were
predicted based on sequences from a model species Drosophila melanogaster and
four aphid species Acyrthosiphon pisum, Myzus persicae, Toxoptera citricida, and
Aphis gosspii. Gene Ontology and KEGG pathway analyses shed light on the
potential functions of these miRNAs in the regulation of genes involved in the
metabolism, development and wing polyphenism of S.
avenae.