Guigen Ma,Fang Yan,Bin Ren,Zhenwan Lu,Hao Xu,Fangxi Wu, Shaofang Li, Daowen Wang, Xueping Zhou, Huanbin Zhou.LbCas12a nuclease-mediated tiling deletion for large-scale targeted editing of non-coding regions in rice.-中国农业科学院植物保护研究所

Guigen Ma,Fang Yan,Bin Ren,Zhenwan Lu,Hao Xu,Fangxi Wu, Shaofang Li, Daowen Wang, Xueping Zhou, Huanbin Zhou.LbCas12a nuclease-mediated tiling deletion for large-scale targeted editing of non-coding regions in rice.

文章来源：Plant Communications 点击数：次发布时间：2024-01-18

Summary

NTD method is the application of LbCas12a in the engineering of rice endogenous gene. Guided by libraries of crRNAs tiling on NCRs of SD1 , 6 mutants with quantitative variations of rice height were generated and analyzed for the association between genotype and phenotype. Totally, NTD is a promising tool for molecular rice breeding.

Plant Communications，IF=10.8

https://doi.org/10.1016/j.xplc.2024.100815

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Guigen Ma,Fang Yan,Bin Ren,Zhenwan Lu,Hao Xu,Fangxi Wu, Shaofang Li, Daowen Wang, Xueping Zhou, Huanbin Zhou.LbCas12a nuclease-mediated tiling deletion for large-scale targeted editing of non-coding regions in rice.