Dianjie Xie, Cong Zhu, Lei Zhang, Yueqiu Liu, Yunxia Cheng, Xingfu Jiang*, Genome-scale analysis of ABC transporter genes and characterization of the ABCC type transporter genes in the oriental armyworm,  Mythimna separata (Walker). International Journal of Biological Macromolecules, 2023,

https:// doi: 10.1016/j.ijbiomac.2023.123915

Abstract

The oriental armyworm  Mythimna separata is a polyphagous, migratory corn pest in China and other Asian countries. Transgenic  Bacillus thuringiensis ( Bt ) corn may effectively control this insect pest. Several reports have suggested that ATP-binding cassette (ABC) transporter proteins may act as receptors that bind  Bt toxins. However, our knowledge about ABC transporter proteins in  M. separata is limited. We identified 43 ABC transporter genes in the  M. separata genome by bioinformatics prediction. Evolutionary tree analysis grouped these 43 genes into 8 subfamilies, ABCA to ABCH. Among the 13 ABCC subfamily genes, the transcript levels of  MsABCC2 and  MsABCC3 were upregulated. In addition, RT-qPCR analyses of these two potentials showed that both were predominantly expressed in the midgut tissue. Knock-down of  MsABCC2 , but not  MsABCC3 , decreased Cry1Ac susceptibility as indicated by increased larval weight and reduced larval mortality. This suggested that  MsABCC2 might play a more important role in Cry1Ac toxicity and that it is a putative Cry1Ac receptor in  M. separata . Together, these findings provide unique and valuable information for future elucidating of the role of ABC transporter genes in  M. separata , which is highly valuable and important for the long-term application of Bt insecticidal protein.

International Journal of Biological Macromolecules, IF="8.025

https://pubmed.ncbi.nlm.nih.gov/36871694/