Scientific Data, 2023, https://doi: 10.1038/s41597-023-02179-y

Abstract

The spotted alfalfa aphid (SAA,  Therioaphis trifolii ) (Hemiptera: Aphididae) is a destructive pest of cultivated alfalfa ( Medicago sativa  L.) that leads to large financial losses in the livestock industry around the world. Here, we present a chromosome-scale genome assembly of  T. trifolii , the first genome assembly for the aphid subfamily Calaphidinae. Using PacBio long-read sequencing, Illumina sequencing, and Hi-C scaffolding techniques, a 541.26 Mb genome was generated, with 90.01% of the assembly anchored into eight scaffolds, and the contig and scaffold N50 are 2.54 Mb and 44.77 Mb, respectively. BUSCO assessment showed a completeness score of 96.6%. A total of 13,684 protein-coding genes were predicted. The high-quality genome assembly of  T. trifolii  not only provides a genomic resource for the more complete analysis of aphid evolution, but also provides insights into the ecological adaptation and insecticide resistance of  T. trifolii .

Scientific Data,IF=9.8

https://pubmed.ncbi.nlm.nih.gov/37173339/