李广悦，中国农业科学院植物保护研究所研究员, 博士生导师, 中国农业科学院“生物杀菌创制与应用”创新团队首席科学家，中国农业科学院青年英才“A”类入选者。2003-2007年就读于河北农业大学生命学院并获得学士学位，2007-2010年就读于中国农业科学院植物保护研究所并获得硕士学位，2010-2014年就读于中国科学院天津工业生物技术研究所并获得博士学位。2014-2017年在德国马普煤炭所Manfred.T.Reetz教授实验室从事博士后研究。2017年11月回国，入职中国农业科学院植物保护研究所。主要从事线虫共生细菌中抗菌活性物质的发掘、代谢途径优化以及代谢途径中关键酶的定向改造等研究。在JACS、ACS Catalysis、Chemical Science、Chemical Communications等学术期刊上发表文章20多篇。
已发表文章
1.Li, G#.; Qin, Y#.; Fontaine, N. T#.; Ng Fuk Chong, M.; Maria‐Solano, M. A.; Feixas, F.; Cadet, X. F.; Pandjaitan, R.; Garcia‐Borràs, M.; Cadet, F., Machine Learning Enables Selection of Epistatic Enzyme Mutants for Stability Against Unfolding and Detrimental Aggregation. ChemBioChem . 2020, 21,1-12. (IF5year ="2.5)
2. 李广悦, 杨秀芬. 嗜线虫致病杆菌抗菌代谢产物 Xcn1 的研究进展. 中国生物防治学报 2020, 36 (1), 1-8.
3. 段佳琪，李琲琲，任杰，邱德文，李广悦*.一种脱氧雪腐镰刀菌烯醇制备工艺的研究.生物技术通报. 2020, 36(10):1-8.
4. Dong, Y.; Li, X.; Duan, J.; Qin, Y.; Yang, X.; Ren, J.; Li, G*. Improving the Yield of Xenocoumacin 1 Enabled by In Situ Product Removal. ACS omega. 2020, 5（32）, 20391–20398. (IF5year ="2.6)
5. Jiaqi Duan, Beibei Li, Youcai Qin, Yijie Dong, Jie Ren and Guangyue Li*. Recent progress in directed evolution of stereoselective monoamine oxidases. Bioresources and Bioprocessing [J]. 2019, 6: 1-11.
6.Guangyue Li, Yijie Dong, and Manfred T. Reetz*. Can Machine Learning Revolutionize Directed Evolution of Selective Enzymes? Adv. Synth. Catal [J]. 2019, 361, 2377 – 2386. (IF5year ="5.029)
7.Yijie Dong#, Peiyuan Yao#, Yunfeng Cui, Qiaqing Wu, Dunming Zhu*, Guangyue Li*and Manfred T. Reetz*. Manipulating the stereoselectivity of a thermostable alcohol dehydrogenase by directed evolution for efficient asymmetric synthesis of arylpropanols. Biological Chemistry [J]. 2019，400(3), 313-321. (IF5year ="3.2)
8.Guangyue Li, Marc Garcia-Borràs, Maximilian J. L. J. Fürst, Adriana Ilie, Marco W. Fraaije, K. N. Houk*and Manfred T. Reetz*. Overriding Traditional Electronic Effects in Biocatalytic Baeyer-Villiger Reactions by Directed Evolution. JACS [J]. 2018, 140, 10464−10472. (IF5year ="14.35)
9.Peiyuan Yao, Peiqian Cong, Rui Gong, Jinlong Li, Guangyue Li, Jie Ren, Jinhui Feng, Jianping Lin, Peter C. K. Lau,* Qiaqing Wu,* and Dunming Zhu*.Biocatalytic Route to Chiral 2-Substituted-1, 2, 3, 4-tetrahydroquinolines Using Cyclohexylamine Oxidase Muteins. ACS Catalysis[J]. 2018, 8, 1648-1652. (IF5year ="10.945).
10.Frédéric Cadet, Nicolas Fontaine, Guangyue Li, Joaquin Sanchis, Matthieu Ng Fuk Chong,Rudy Pandjaitan, Iyanar Vetrivel , Bernard Offmann & Manfred T. Reetz. A machine learning approach for reliable prediction of amino acid interactions and its application in the directed evolution of enantioselective enzymes. Scientific reports [J]. 2018, 8:16757. (IF5year ="4.12)
11.Ge Qu, Richard Lonsdale, Peiyuan Yao, Guangyue Li, Beibei Liu, Manfred T. Reetz,* and Zhoutong Sun*. Methodology Development in Directed Evolution: Exploring Options when Applying Triple‐Code Saturation Mutagenesis. ChemBioChem[J]. 2018, 19, 239-246. (IF5year ="2.751).
12.Guangyue Li#, Peiyuan Yao#, Rui Gong#, Jinlong Li, Pi Liu，Richard Lonsdale, Qiaqing Wu, Jianping Lin*，Dunming Zhu* and Manfred T. Reetz*. Simultaneous engineering of an enzyme’s entrance tunnel and active site: The case of monoamine oxidase MAO-N. Chemical Science [J]. 2017, 8，4093-4099. (IF5year="8.733)
13.Guangyue Li, Miguel A. Maria-Solano, Adrian Romero-Rivera, Sílvia Osuna* and Manfred T. Reetz*. Inducing High Activity of a Thermophilic Enzyme at Ambient Temperatures by Directed Evolution. Chemical Communications [J]. 2017, 53，9454-9457. (IF5year ="6.238)
14.Guangyue Li, M.J. L. J. Fürst, H. R. Mansouri, A. K. Ressmann, A. Ilie, F. Rudroff, M. D. Mihovilovic*, M.W. Fraaije* and M. T. Reetz*. Manipulating the stereoselectivity of the thermostable Baeyer–Villiger monooxygenase TmCHMO by directed evolution. Organic & Biomolecular Chemistry [J]. 2017，15，9824-9829. (IF5year ="3.56)
15.Guangyue Li, Jianbo Wang and Manfred T. Reetz*. Biocatalysts for the pharmaceutical industry generated by structure-guided directed evolution ofstereoselective enzymes. Bioorganic & Medicinal Chemistry [J]. 2017, 7(26), 1241-1251 (IF5year="2.88)
16.Jian-bo Wang, Guangyue Li, Manfred T. Reetz*. Enzymatic site-selectivity enabled by structure-guided directed evolution. Chemical Communications [J]. 2017, 53, 3916-3928. (IF5year ="6.238)
17.Guangyue Li#, Hui Zhang#, Zhoutong Sun, Xinqi Liu and Manfred T. Reetz*. Multiparameter Optimization in Directed Evolution: Engineering Thermostability, Enantioselectivity, and Activity of an Epoxide Hydrolase. ACS Catalysis [J]. 2016, 6(6), 3679−3687. (IF5year ="10.945)
18.Guangyue Li and Manfred T. Reetz*. Learning Lessons from Directed Evolution of Stereoselective Enzymes. Org. Chem. Front [J]. 2016. 3, 1350-1358 (IF5year ="4.96)
19.Guangyue Li#, Peiyuan Yao#, Peiqian Cong, Jie Ren, Lei Wang, Jinhui Feng, Peter C.K. Lau, Qiaqing Wu*,and Dunming Zhu*. New recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates. Scientific reports [J]. 2016，6：24973. (IF5year ="4.847)
20.Zhoutong Sun†, Guangyue Li†, Adriana Ilie†, Manfred T. Reetz *. Exploring the substrate scope of mutants derived from the robust alcohol dehydrogenase TbSADH. Tetrahedron Letters[J]. 2016. 57, 3648-3651. (IF5year ="2.02).
21.Zhoutong Sun, Richard Lonsdale, Adriana Ilie, Guangyue Li, Jiahai Zhou, and Manfred T. Reetz. Catalytic Asymmetric Reduction of Difficult-to-Reduce Ketones:Triple-Code Saturation Mutagenesis of an Alcohol Dehydrogenase. ACS Catalysis [J]. 2016, 6(3)， 1598−1605. (IF5year ="10.945)
22.Zhoutong Sun, Richard Lonsdale, Lian Wu, Guangyue Li, Aitao Li, Jianbo Wang, Jiahai Zhou, and Manfred T. Reetz. Structure-Guided Triple-Code Saturation Mutagenesis: Efficient Tuning of the Stereoselectivity of an Epoxide Hydrolase. ACS Catalysis [J]. 2016, 6， 1590−1597. (IF5year ="10.945)
23.Zhoutong Sun, Richard Lonsdale, Guangyue Li and Manfred T. Reetz. Comparing Different Strategies in Directed Evolution of Enzyme Stereoselectivity: Single versus Double Code Saturation Mutagenesis. Chembiochem [J]. 2016, 17， 1865-1872. (IF5year ="2.751)
24.Guangyue Li#, Jie Ren#, Peiyuan Yao,Yitao Duan, Hailing Zhang, Qiaqing Wu, Jinhui Feng, Peter CK Lau*, Dunming Zhu*. Deracemization of 2-Methyl-1, 2, 3, 4-tetrahydroquinoline Using Mutant Cyclohexylamine Oxidase Obtained by Iterative Saturation Mutagenesis. ACS Catalysis [J]. 2014, 4，903-908. (IF5year ="9.174)
25.Guangyue Li, Jie Ren, Hiroaki Iwaki, Dalong Zhang, Yoshie Hasegawa, Qiaqing Wu, Jinhui Feng, Peter C. K. Lau* and Dunming Zhu*. Substrate profiling of cyclohexylamine oxidase and its mutants reveals new biocatalytic potential in deracemization of racemic amines. Applied Microbiology and Biotechnology [J]. 2014, 98(4)，1681-1689. (IF5year ="3.848)
26.Guangyue Li, Jie Ren, Qiaqing Wu*, Jinhui Feng, Dunming Zhu*, Yanhe Ma. Identification of a marine NADPH-dependent aldehyde reductase for chemoselective reduction of aldehydes. Journal of molecular catalysis B, Enzymatic [J], 2013, 90，17-22. (IF5year ="2.786)
27.Li GuangYue，Yang XiuFen，Zeng HongMei，Mao JianJun，Liu Hua，Zhang YunHua，Qiu, DeWen* Stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by PeaT1. Science China Life Sciences [J], 2010, 53(12)，1410-7. (近五年平均IF=2.88)
28.梁颖博, 李泽, 邱德文, 曾洪梅, 李广悦, 杨秀芬. 本生烟响应蛋白激发子 PevD1 的差异表达基因鉴定与分析. 中国农业科学, 2019，52(21), 3794-3805.
29.刘 权, 李广悦, 曾洪梅, 杨秀芬, 邱德文. 微生物蛋白激发子PeaT1的获得及诱导水稻抗旱性的初步研究. 中国农业科技导报, 2009, 11( 3)， 51- 55.
30.刘权，李广悦，曾洪梅，杨秀芬，邱德文．蛋白激发子PeaT1的高密度发酵及生理功能检测．生物技术通报，2009，8，162-165．
